Open AccessOptimization of Crude Oil Biodegradation of Fungi Isolated from Refinery Effluent Site using Response Surface Methodology
Author(s) -
U.C. Odili,
F.B. Ibrahim,
E.M. Shaibu-modagbe,
H.I. Atta
Publication year - 2021
Publication title -
the nigerian journal of technological development
Language(s) - English
Resource type - Journals
ISSN - 2437-2110
DOI - 10.4314/njtd.v17i4.3
Subject(s) - bioremediation , response surface methodology , effluent , refinery , oil refinery , pulp and paper industry , biodegradation , hydrocarbon , petroleum , microbial consortium , contamination , environmental science , chemistry , environmental chemistry , environmental engineering , microorganism , chromatography , geology , biology , bacteria , ecology , organic chemistry , engineering , paleontology
The activities involved in the production and exploration of crude oil has constantly polluted the environment. This study investigated the ability of an indigenous fungus to utilize petroleum hydrocarbon. Response Surface Methodology was used to optimize the effects of pH, microbial concentration (spores/ml), and contact time (days) on the crude oil removal efficiency in refinery effluent. Monocillium sp. was isolated and used for the treatment of refinery effluent due to its predominance in the contaminated soil. Twenty experimental runs were analyzed to determine the effect of pH, microbial concentration and contact time on the oil removal efficiency. From theexperimental results obtained, a maximum oil removal efficiency of 98.42 % was achieved at a pH of 6.5, contact time of 14 days, and a microbial concentration of 3 spores/ml. The results obtained showed the percentage of crude oil removal in the effluent sample increased with an increase in time. Optimization of the experimental result was achieved at a removal efficiency of 98.59 %, a contact time of 13.96 days, a pH of 6.85, and a microbial concentration of 3.01 spores/ml. The findings of this study revealed that Monocillium sp. is a viable hydrocarbon degrader, and can be used in the bioremediation of petroleum contaminated environments.
Keywords: Response surface, optimization, bioremediation, hydrocarbon, removal efficiency, Monocillium sp.