Open AccessStudies on Analgesic and Anti-Inflammatory Activities of Aerial Parts of Tephrosia Bracteolata GUILL. and PERR. (Fabaceae) in Rodents
Author(s) -
Abdul Sadam,
S.M. Abdullahi,
U. U. Pateh,
Aishatu Shehu,
L.O. Bakare
Publication year - 2021
Publication title -
nigerian journal of pharmaceutical research
Language(s) - English
Resource type - Journals
eISSN - 2635-3555
pISSN - 0189-8434
DOI - 10.4314/njpr.v16i2.4s
Subject(s) - phytochemical , analgesic , traditional medicine , median lethal dose , pentazocine , anti inflammatory , fabaceae , pharmacology , edema , medicine , ibuprofen , glibenclamide , chemistry , toxicity , botany , biology , diabetes mellitus , endocrinology
Background: Tephrosia bracteolata is a widespread shrub belonging to the family (Fabaceae) and genus Tephrosia. It is traditionally used for treating rheumatic pains, dropsy and stomach ache.Objectives: In view of the ethnomedicinal claim and the continuous search for new medicinal agents, the phytochemical constituents, analgesic and anti-inflammatory activities of chloroform fraction (CF) of the methanol extract of Tephrosia bracteolata in mice and rats was evaluated.Methods: Preliminary phytochemical screening was conducted using standard method. Analgesic activity of CF (100, 200 and 400 mg/kg body weight orally) was investigated using acetic acid-induced writhing test and thermally induced pain model in mice. Additionally, anti-inflammatory activity was tested by carragenaan-induced paw edema in rats.Results: Phytochemical screening revealed the presence of alkaloids, triterpenes and flavonoids. The oral LD50 of CF was above 2000 mg/kg body weight. CF significantly (p<0.05) and dose dependently reduced the number of writhes with percentage inhibition of 47.76 48.41 and 72.6 % at dose of 100, 200 and 400mg/kg respectively. CF also significantly (p<0.05) and dose dependently increased the mean reaction time. At dose of 400 mg/kg, CF at 60 and 90 minutes exhibited greater activity when compared to the standard agent pentazocine. CF(200 and 400 mg/kg) at times 3, 4 and 5 hours significantly (p<0.05) decreased the paw edema in rats when compare with the ibuprofen treated group.Conclusions: The chloroform fraction of the methanol crude extract of Tephrosia bracteolata possesses analgesic and anti-inflammatory activities.