Open AccessMushroom-mediated delignification of agricultural wastes for bio-ethanol production
Author(s) -
A.A. Elimam,
Pheobe Queen Akoh,
S. Saliman,
Eromosele Ighalo
Publication year - 2021
Publication title -
nigerian journal of biotechnology
Language(s) - English
Resource type - Journals
ISSN - 0189-1731
DOI - 10.4314/njb.v38i1.16
Subject(s) - pleurotus ostreatus , sawdust , mushroom , biofuel , husk , chemistry , food science , biomass (ecology) , ethanol fuel , pulp and paper industry , lignin , pleurotus , straw , fermentation , microbiology and biotechnology , botany , agronomy , biology , organic chemistry , engineering , inorganic chemistry
Biological pretreatment is a cost-effective method of delignifying lignocellulosic biomass, making it less recalcitrant to hydrolysis into fermentable sugars. In this study, selected agricultural wastes were pretreated with mushrooms (Lentinus squarrosulus and Pleurotus ostreatus) to delignify them for bioethanol production. The substrates were supplemented with 0.2 % CaCO3, inoculated with 12 % (w/w) L. squarrosulus and Pleurotus ostreatus spawns and incubated at 25 oC for 21 days. The highest lignin removal and highest bioethanol yield of 77.45 % and 13.98 % were obtained from bean husks pretreated with L. squarrosulus. Similarly, 64.29 % and 60.92 % lignin were removed from the Pleurotus ostreatus-pretreated banana leaves and sawdust, respectively, while 12.08 % and 13.05 % bio-ethanol yields were recorded, respectively. These findings demonstrate that affordable and straightforward mushroom delignification of abundant and cheap biomass can improve hydrolysis outcomes, thus easing bioethanol production.