Open AccessMicrosatellite markers-based characterisation of elephant grass (<i>Pennisetum purpureum</i>) harvested from selected locations in South-West Nigeria
Author(s) -
O. A. Okukenu,
Amos Afolarin Olajide,
P. A. Dele,
Mathew Wheto,
B. T. Akinyemi,
A. O. Jolaosho,
B.O. Jokosenumi,
T.J. Shonde
Publication year - 2020
Publication title -
nigerian journal of biotechnology
Language(s) - English
Resource type - Journals
ISSN - 0189-1731
DOI - 10.4314/njb.v37i1.4
Subject(s) - pennisetum purpureum , genetic diversity , biology , microsatellite , pennisetum , population , botany , veterinary medicine , allele , genetics , dry matter , gene , demography , sociology , medicine
This study was carried out to characterise Pennisetum purpureum harvested from some selected locations in S outh-W estern Nigeria using microsatellite markers. Leaf parts of growing young elephant grass (Pennisetum purpureum) were harvested and immediately preserved in ethanol solution before DNA extraction. Two (2) SSR primers (CTM59 and Xtxp278) were used to assess genetic diversity in Pennisetum purpureum. The result shows that 72% of the molecular variations in the elephant grass exists within the population with 28% among the population; there were no unique characteristics among the Nine (9) populations. Nei genetic index ranged from 0.067 (lowest) observed between Isokan and Odeda populations to 0.158 (highest), between Ifedore and Ikoyi Populations. Morphological characterization showed moderate diversity with two major clusters and one minor cluster.
Keyword: Elephant grass; cultivars; locations; markers