Open AccessSDS-Page characterization of some elite cowpea (Vigna unguiculata L. Walp) varieties
Author(s) -
Atanda Samuel Oladejo,
Abolade Oluremi Bolaji,
I. O. Obisesan,
Ofelia Galman Omitogun
Publication year - 2020
Publication title -
nigerian journal of biotechnology
Language(s) - English
Resource type - Journals
ISSN - 0189-1731
DOI - 10.4314/njb.v36i2.6
Subject(s) - dendrogram , vigna , biology , polyacrylamide gel electrophoresis , upgma , horticulture , veterinary medicine , genotype , genetic diversity , genetics , gene , biochemistry , medicine , population , demography , sociology , enzyme
The shortcomings of genotype x environment interaction necessitated the use of molecular methods in characterizing many plant species and in determining their phylogenetic relationships. In this study, some selected cowpea lines (27 varieties) from Obafemi Awolowo University, Ile – Ife, the Institute of Agricultural Research (IAR), Samaru, Kaduna and Genetic Resource Centre, IITA, Ibadan were characterized using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) profiling. The protein banding profiles of the 27 cowpea varieties were scored and subjected to cluster analysis using Ward's minimum-variance method (WMVM) for dendrogram grouping. The dendrogram generated from the SDS-PAGE profiles grouped the varieties into seven clusters at 52% similarity coefficient. Hence, the biochemical characterization revealed more precise discrimination among the 27 cowpea varieties studied.
Keywords: Cowpea, electrophoretic banding profiles, dendrogram grouping, total proteins