Open AccessIn vitro antiplasmodial evaluation of ethanolic and n-hexane extracts of Parinari curatellifolia stem bark
Author(s) -
Babasoji Percy Omoniwa,
Kunle Okaiyeto,
D. O. Omoniwa,
Olumuyiwa A. Olorunyomi
Publication year - 2021
Publication title -
journal of pharmacy and biore sources
Language(s) - English
Resource type - Journals
ISSN - 0189-8442
DOI - 10.4314/jpb.v18i2.3
Subject(s) - parasitemia , traditional medicine , plasmodium falciparum , biology , bark (sound) , artemether , malaria , medicine , artemisinin , immunology , ecology
Parinari curatellifolia and other Parinari species are used traditionally in many parts of Africa as a remedy for malaria among other diseases. To ascertain this folkloric claim, the antiplasmodial potential of ethanol extract of Parinari curatellifolia stem bark (EEPCSB) and n-hexane extract of Parinari curatellifolia stem bark (HEPCSB) on Plasmodium falciparum was studied. Parasites were grown in a 96-well plate containing Roswell Park Memorial Institute-1640. The wells were grouped into: control (untreated), artemether-treated, EEPCSB-treated and HEPCSBtreated groups. Treatments were administered to the tune of 10, 20, 40 and 80 μg/ml. Parasitemia was observed by microscopy after 24, 48 and 72h of incubation. EEPCSB and HEPCSB elicited dose and duration-dependent reduction (p 0.05) in plasmodium lactate dehydrogenase activity of EEPCSB-treated and artemether-treated groups. Findings from this study show that extracts of P. curatellifolia stem bark, especially EEPCSB, demonstrated excellent inhibitory activities against P. falciparum and can be a good source of compounds for the development of novel antimalarial drugs.
Keywords: Parinari curatellifolia; Extracts; Plasmodium falciparum; Parasitemia; Antiplasmodial