Open AccessEffects of green tea (Camellia sinensis) on paracetamol-induced oxidative stress markers in Wistar rats
Author(s) -
E.P. Ezeja,
N. Onuoha,
E.A. Ufere
Publication year - 2022
Publication title -
journal of dietitians association of nigeria
Language(s) - English
Resource type - Journals
eISSN - 2635-3326
pISSN - 2141-8209
DOI - 10.4314/jdan.v12i1.5
Subject(s) - malondialdehyde , green tea extract , oxidative stress , camellia sinensis , glutathione peroxidase , superoxide dismutase , catalase , green tea , antioxidant , ascorbic acid , glutathione , chemistry , vitamin c , food science , traditional medicine , medicine , biochemistry , biology , botany , enzyme
Background: Green tea is a good source of phenolic compounds which are known to possess antioxidant properties. Although there have been researches on the effect of fresh green tea leaves on oxidative stress, there is paucity of data on processed green teas consumed in Nigeria.
Objective: This study was conducted to determine the effect of green tea on oxidative stress biomarkers in Adult male Wister rats.
Methods: Serum malondialdehyde (MDA) level, catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), vitamins A, C and E activity of rats treated with green tea extract (GTE) were determined using standard methods. Thirty two (32) rats were divided into six groups of five rats each. Group 1 fed only on normal rat chow. All groups except group I were oxidative stress induced with 1500mg/kg body weight (bw) of paracetamol orally for three consecutive days. Group 2 did not receive any treatment. Group 3 was treated with ascorbic acid (20mg/kg bw) for seven consecutive days while groups 4 to 6 were treated with 50, 100, 200 mg/kg bw of GTE respectively for seven consecutive days.
Results: There was a significant (p 0.05) difference among all the groups. The vitamin A level of the groups 4 to 5 showed a significant (p<0.05) decrease compared with groups 1 to 3. However, vitamin C and E levels of groups 5 increased significantly (p<0.05) compared with group 2.
Conclusion: GTE consumption at 50, 100, 200 mg/kg bw for seven consecutive days caused reduction in serum MDA level and increased serum enzymatic and non-enzymatic antioxidants in rats.