Open AccessIsolation and NMR Characterization of Ursane-Type Triterpenoid from the Leaves of Peperomia pellucida
Author(s) -
J.C. Ibe–Diala,
Okenwa Uchenna Igwe,
C. Friday,
Ugochukwu Clinton Akwada
Publication year - 2021
Publication title -
journal of applied science and environmental management
Language(s) - English
Resource type - Journals
eISSN - 2659-1499
pISSN - 2659-1502
DOI - 10.4314/jasem.v25i3.13
Subject(s) - piperaceae , triterpenoid , chromatography , dept , extraction (chemistry) , chemistry , traditional medicine , botany , stereochemistry , biology , piper , medicine
Peperomia pellucida is a member of the Piperaceae family. Extraction of plant material was carried out by Soxhlet extraction method using hexane and ethylacetate as solvents respectively. The extract was concentrated using a rotary evaporator, followed by isolation and purification using column and thin layer chromatographic techniques. Fraction C20 showed a clearly defined single spot with Rf value of 0.51. Using 1HNMR, 13C-DEPT, COSY, HSQC and HMBC and by comparison with literature values, the structure of the compound was established as an Ursane-type triterpenoid. The use of P. pellucida in ethnomedicine for the treatment of various ailments could be attributed to the Ursane-type triterpenoid and other bioactive chemical compounds present in the plant.
Keywords: Peperomia pellucida, extraction, isolation, chromatographic techniques, ursane-type triterpenoid