Isolation and NMR Characterization of Ursane-Type Triterpenoid from the Leaves of Peperomia pellucida | Zendy

J.C. Ibe–Diala | Zendy; Okenwa Uchenna Igwe | Zendy; C. Friday | Zendy; Ugochukwu Clinton Akwada | Zendy

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Isolation and NMR Characterization of Ursane-Type Triterpenoid from the Leaves of Peperomia pellucida

Author(s) -

J.C. Ibe–Diala,

Okenwa Uchenna Igwe,

C. Friday,

Ugochukwu Clinton Akwada

Publication year - 2021

Publication title -

journal of applied science and environmental management

Language(s) - English

Resource type - Journals

eISSN - 2659-1499

pISSN - 2659-1502

DOI - 10.4314/jasem.v25i3.13

Subject(s) - piperaceae , triterpenoid , chromatography , dept , extraction (chemistry) , chemistry , traditional medicine , botany , stereochemistry , biology , piper , medicine

Peperomia pellucida is a member of the Piperaceae family. Extraction of plant material was carried out by Soxhlet extraction method using hexane and ethylacetate as solvents respectively. The extract was concentrated using a rotary evaporator, followed by isolation and purification using column and thin layer chromatographic techniques. Fraction C20 showed a clearly defined single spot with Rf value of 0.51. Using 1HNMR, 13C-DEPT, COSY, HSQC and HMBC and by comparison with literature values, the structure of the compound was established as an Ursane-type triterpenoid. The use of P. pellucida in ethnomedicine for the treatment of various ailments could be attributed to the Ursane-type triterpenoid and other bioactive chemical compounds present in the plant. Keywords: Peperomia pellucida, extraction, isolation, chromatographic techniques, ursane-type triterpenoid

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