Open AccessIsolation and Characterization of Larvicidal Phenolic Acids from Kotschya thymodora Leaves
Author(s) -
Innocent John Daniel,
Ester Innocent,
Joseph Sempombe,
Veronica Mugoyela,
Torgils Fossen
Publication year - 2020
Publication title -
journal of applied science and environmental management
Language(s) - English
Resource type - Journals
eISSN - 2659-1499
pISSN - 2659-1502
DOI - 10.4314/jasem.v24i8.26
Subject(s) - vanillic acid , anopheles gambiae , phytochemical , culex quinquefasciatus , biology , anopheles , population , traditional medicine , protocatechuic acid , botany , anopheles stephensi , larva , malaria , aedes aegypti , food science , biochemistry , medicine , antioxidant , environmental health , immunology
Malaria is a vector borne disease responsible for high morbidity, mortality and poverty in many tropical and subtropical countries. The disease is transmitted through a bite from an infected female Anopheles mosquito, amongst which Anopheles gambiae s.s serves as the most prevalent vector. The control of An. gambiae s.s population can therefore lead to a reduction in malaria spreading. Previous studies have reported the crude extracts of Kotschya thymodora to be active against the larvae of An. gambiae s.s and Culex quinquefasciatus. In this report the phytochemical study on the crude aqueous ethanolic extract of K. thymodora leaves led to isolation of vanillic acid (1) and protocatechuic acid (2). The structures of these compounds and mosquitoes larvicidal activity against An. gambiae s.s were established by using spectroscopic techniques and WHO protocol of 1996 respectively. The two phenolic acids exhibited a moderate mosquito larvicidal activity with LC50 of 77.35 μg/mL (vanillic acid, 1) and 62.4 μg/mL (protocatechuic acid, 2) after 48 hrs exposure time. This is the first report on the isolation of the two phenolic acids from plants belonging to the genus Kotschya and their larvicidal potential against An. gambiae s.s.
Keywords: larvicidal activity, vanillic acid, protocatechuic acid, Anopheles gambiae s.s and Kotschya thymodora