Open AccessIsolation of lupeol from the stem bark of Leptadenia hastata (Pers.) Decne
Author(s) -
Maria M. Mailafiya,
U. U. Pateh,
H. N. A. Hassan,
MI Sule,
A.H. Bila,
Tabitha Lubo Musa,
Vallada Atinga,
J.I. Achika
Publication year - 2020
Publication title -
journal of applied science and environmental management
Language(s) - English
Resource type - Journals
eISSN - 2659-1499
pISSN - 2659-1502
DOI - 10.4314/jasem.v24i10.19
Subject(s) - lupeol , chloroform , phytochemical , chemistry , maceration (sewage) , chromatography , methanol , column chromatography , butanol , hexane , traditional medicine , organic chemistry , ethanol , materials science , biochemistry , medicine , composite material
Dried stem bark powder of Leptadania hastata was subjected to maceration with methanol to afford crude methanol extract, which was partitioned with n-hexane, ethylacetate, chloroform and n- butanol to afford different their respective fractions. Extensive phytochemical screening of the n-hexane fraction using column chromatography resulted to the isolation of a white solid substance. The substance was identified as of lupeol using IR, 1D – NMR, 2D – NMR data and by comparison with reference spectral data.
Keywords: Leptadenia hastata, Isolation, Lupeol, 1D – NMR, 2D – NMR