Open AccessHepatoprotective effects of ethanol extract of Caesalpiniabonduc against carbon tetrachloride induced hepatotoxicity in Albino Rats
Author(s) -
A.E. Ubhenin,
Ighodaro Igbe,
Fatima Amin Adamude,
Abiodun Falodun
Publication year - 2016
Publication title -
journal of applied science and environmental management
Language(s) - English
Resource type - Journals
eISSN - 2659-1499
pISSN - 2659-1502
DOI - 10.4314/jasem.v20i2.22
Subject(s) - carbon tetrachloride , antioxidant , chemistry , ccl4 , catalase , pharmacology , glutathione peroxidase , glutathione , biochemistry , ethanol , bilirubin , in vivo , toxicity , enzyme , biology , medicine , organic chemistry , microbiology and biotechnology
The present study was carried out to evaluate the Acute toxicity, hepatoprotective and in-vivo antioxidant activities of ethanolic extract of Caesalpinia bonduc leaf on carbon tetrachloride (CCl4) induced liver damage using Swiss albino rats . The ethanolic extract of the plant of C. bonduc were suspended in 5 % tragacanth and then administered orally at doses of 250 and 500 mg/kg body weight for fourteen days before intraperitoneally injection of Carbon tetrachloride (CCl4) at dose of 2 mL/kg body weight. The plant extracts at 250 and 500mg/kg b.wt showed a remarkable hepatoprotective and invivo antioxidant activities against carbon tetrachloride CCl4 – induced hepatotoxity judged from the serum marker enzymes .The CCl4 induced significant increase in aspartate amino transferase( AST), alanine amino transferase (ALT), alkaline phophatase (ALP), total bilirubin, and malondyaldehyde (MDA) with a reduction of total protein, catalase, and glutathione peroxidase . Treatment of rats with different doses of plant extract (250 and 500 mg/kg b.wt.) significantly (P< 0.001) altered serum maker enzymes and antioxidant levels to near normal levels. The study suggests that C. Bonduc specifically chloroform and ethyl acetate fraction may be good sources of natural antioxidant and hepatoprotective substance.Keywords: Ceasalpiniabonduc, hepatoprotective, intraperitoneally , antioxidant