Open AccessLevels of induced free proline in chelated and unchelated hydroponically grown lettuce (<i>Lactuca sativa L.</I>)
Author(s) -
M.S. Dagari,
M.S. Musa,
F.M. Maude
Publication year - 2020
Publication title -
bayero journal of pure and applied sciences
Language(s) - English
Resource type - Journals
ISSN - 2006-6996
DOI - 10.4314/bajopas.v12i1.66s
Subject(s) - lactuca , proline , chelation , edds , chemistry , horticulture , cadmium , botany , heavy metals , biochemistry , amino acid , biology , inorganic chemistry , environmental chemistry , phytoremediation , organic chemistry
It is well described that under stress conditions many plant species accumulates proline as adaptive response to adverse conditions. In this study, eight weeks old Lactuca sativa L. seedlings were transplanted in nutrient solution containing various doses (0.000, 0.0025, 0.005, 0.0075, and 0.025mg/L) of Pb2+ and Cd2+ and constant concentration of EDDS (0.0025M). The effects of these treatments of Pb2+, Cd2+, combination of Pb2+ + Cd2+and with or without EDDS (Ethylene diamine disuccinate) were compared based on the level of stress induced to the seedlings. Free proline contents induced due to stress by uptake of Pb2+and Cd2+ were investigated in both unchelated and chelated treatment. The results obtained showed that the effect of increasing concentration of Pb2+ in hydroponic solution leads to decrease in the level of stress induced to the seedlings by EDDS while the lower the Cd2+ concentration in the hydroponic solution the more the level of stress induced by EDDS to the plant. The highest level of stress caused to the seedlings by unchelated and chelated Pb2+ solution are 68.294±5.642; 72.681±14.740 µmolg-1 respectively while that of Cd2+ are 28.962±8.703; 48.263±2.455 µmolg-1 respectively. From this, it is evident that Pb2+induced more stress to the plants than Cd2+ in the presence and absence of EDDS (p<0.05).
Keywords: Proline, Ethylenediaminedisuccinic Acid (EDDS), Lead, Cadmium, Lettuce.