Open AccessApoptosis Inducing Effect Of Andrographolide On TF-47 Human Breast Cancer Cell Line
Author(s) -
Sukardiman Harjotaruno,
Aty Widyawaruyanti,
Sismindari,
Noor Cholies Zaini
Publication year - 2008
Publication title -
african journal of traditional complementary and alternative medicines
Language(s) - English
Resource type - Journals
eISSN - 2505-0044
pISSN - 0189-6016
DOI - 10.4314/ajtcam.v4i3.31228
Subject(s) - andrographolide , andrographis paniculata , apoptosis , acanthaceae , cell culture , dna fragmentation , mcf 7 , cancer research , breast cancer , cell , fragmentation (computing) , human breast , chemistry , cancer , programmed cell death , biology , microbiology and biotechnology , medicine , traditional medicine , pharmacology , pathology , biochemistry , genetics , ecology , alternative medicine
Andrographolide isolated from Andrographis paniculata Ness (Acanthaceae) at 0.35 mM, 0.70 mM and 1.40 mM induced DNA fragmentation and increased the percentage of apoptotic cells when TD-47 human breast cancer cell line was treated for 24, 48 and 72 h. The results demonstrated that andrographolide can induce apoptosis in TD-47 human breast cancer cell line in a time and concentration-dependent manner by increase expression of p53, bax, caspase-3 and decrease expression of bcl-2 determined by immunohistochemical analysis.