Virulence genes and antimicrobial resistance pattern in Proteus mirabilis strains isolated from patients attended with urinary infections to Tertiary Hospitals, in Iran | Zendy

Azardokht Tabatabaei | Zendy; Khadijeh Ahmadi | Zendy; Alireza Namazi Shabestari | Zendy; Nastaran Khosravi | Zendy; Ali Badamchi | Zendy

Open Access

Virulence genes and antimicrobial resistance pattern in Proteus mirabilis strains isolated from patients attended with urinary infections to Tertiary Hospitals, in Iran

Author(s) -

Azardokht Tabatabaei,

Khadijeh Ahmadi,

Alireza Namazi Shabestari,

Nastaran Khosravi,

Ali Badamchi

Publication year - 2021

Publication title -

african health sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.391

H-Index - 44

eISSN - 1729-0503

pISSN - 1680-6905

DOI - 10.4314/ahs.v21i4.22

Subject(s) - proteus mirabilis , microbiology and biotechnology , virulence , cefotaxime , nalidixic acid , ampicillin , antibiotic resistance , cephalosporin , multiple drug resistance , antibiotics , biology , antimicrobial , medicine , gene , escherichia coli , genetics

Background: Proteus mirabilis is a frequent reason for catheter-associated urinary tract infections (UTIs). The aim of this study was to identify virulence genes and antimicrobial resistance patterns in P. mirabilis strains isolated from patients whoattended a tertiary hospital in Iran. Methods: In this study, 100 P. mirabilis strains from urine samples were isolated. These isolated strains were identified by biochemical and PCR-based tests, and their antibiotic resistance was profiled through a standard procedure using 14 antibiotics.PCR assays were used to detect virulence-related genes in P. mirabilis strains. The biofilm formation of each P. mirabilis strain was examined. Results: Of the 100 P. mirabilis isolates, 16 (16%) were multidrug-resistant. High resistance was observed against cotrimoxazole (97%), nalidixic acid (93%), cefotaxime (77%), and amoxicillin (62%). Sixty of the 100 isolates showed resistance against extended-spectrum cephalosporins. The prevalence rates of the genes related to the virulence factors in this study were mrpH (100%), ucaA (91%), hpmA (94%), zapA (95%), ptaA (100%), ureG (100%), pmfA (100%), fliC (97%), and mrpA (90%) using PCR method. Strong biofilm formation was observed in 20% (5/25) of the strains isolated fromnon-catheterized samples and 80% (20/25) of strains isolated from catheterized samples. Conclusions: Resistance to antibiotics and the prevalence of pathogenicity genes are high in Proteus mirabilis strains iolated from UTIs. Keywords: Antibiotic resistance; Proteus mirabilis; biofilm; virulence factors.

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