Open AccessAssociation of Rs61764370 polymorphism within let-7 microRNA-binding site with lung cancer in Iranian population
Author(s) -
Neda Farokhzad,
Sayed Mostafa Hosseini,
Houri Edalat,
Morteza Sadeghi
Publication year - 2020
Publication title -
african health sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.391
H-Index - 44
eISSN - 1729-0503
pISSN - 1680-6905
DOI - 10.4314/ahs.v20i3.35
Subject(s) - lung cancer , genotype , medicine , kras , allele , oncology , case control study , population , genetics , gene , cancer , biology , colorectal cancer , environmental health
Polymorphisms within miRNAs binding sites are associated with miRNAs function. The aim of this study was to investigate the relationship between rs61764370 polymorphism within let-7 miRNA binding site in KRAS gene and the risk of lung cancer in Iranian population.
Methods: This case-control study was conducted with 100 lung cancer patients and 100 healthy persons. The rs61764370 polymorphism was analyzed using PCR-RFLP technique and direct sequencing.
Results: We found a significant relationship between rs61764370 (T / G) polymorphism and lung cancer risk, the GT gen- otype (OR: 6.25; 95% CI = 2.605-15.00; P= 0.000) and G allele (OR: 5.25; 95% CI = 2.259–12.208; P= 0.000) were signifi- cantly associated with an increased risk of lung cancer.
Conclusion: According to our findings, there is a significant relationship between the KRAS rs61764370 polymorphism and lung cancer risk in Iranian population and this polymorphism may be used as a marker in detection of lung cancer in the future.
Keywords: Polymorphism; KRAS; lung cancer; Let-7.