Open AccessSingle tube allele specific PCR: a low cost technique for molecular screening of sickle cell anaemia in Nigeria
Author(s) -
Emuejevoke T Toye,
Guido van Marle,
Wendy Hutchins,
Olayinka Abgabiaje,
Joy Okpuzor
Publication year - 2018
Publication title -
african health sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.391
H-Index - 44
eISSN - 1729-0503
pISSN - 1680-6905
DOI - 10.4314/ahs.v18i4.20
Subject(s) - medicine , allele , polymerase chain reaction , pediatrics , genetics , gene , biology
Sickle cell anaemia (SCA) is a hereditary blood disorder caused by a single mutation in the haemoglobin gene. The disease burden of SCA is highest in Nigeria. The allele specific polymerase chain reaction (ASPCR) method is applicable for the direct detection of known single nucleotide polymorphisms (SNPs).