Open AccessBiotransformasi (−)-Epigalokatekin-3- O -galat Menjadi (−)-2 R ,3 S -Dihidromirisetin oleh Fungi Endofit Diaporthe sp. Isolat E dari Tumbuhan Teh
Author(s) -
Andria Agusta
Publication year - 2007
Publication title -
hayati journal of biosciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.305
H-Index - 17
eISSN - 2086-4094
pISSN - 1978-3019
DOI - 10.4308/hjb.14.4.150
Subject(s) - camellia sinensis , plant use of endophytic fungi in defense , sephadex , fungus , silica gel , biotransformation , botany , fermentation , chemistry , biology , chromatography , biochemistry , enzyme
Endophytic fungi have been reported possess an interesting ability to mimic their host plant metabolites. Several fungi also show their specific capability to biotransform the chemical constituents of the host plant. The endophytic fungus Diaporthe sp. E isolate obtained from young stem of a tea plant (Camellia sinensis) show their unique capability to biotransform (−)-epigallocatechin-3-O-gallate [(−)-EGCG] into a major product in glucose-peptone-yeast extract medium that incubated under dark condition at 27 °C for 48 h. The major biotransformation product were isolated and purified through column chromatography techniques using Sephadex LH-20 and silica gel. The chemical structure of the major product were elucidated as (−)-2R,3S-dihydromyricetin based on their IR, FAB-MS, 1D- and 2D-NMR spectra