Open AccessDeteksi Molekular Bakteri Endofit pada Jaringan Planlet Tebu
Author(s) -
Wiwik Eko Widayati,
Jaka Widada,
Joedoro Soedarsono
Publication year - 2007
Publication title -
hayati journal of biosciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.305
H-Index - 17
eISSN - 2086-4094
pISSN - 1978-3019
DOI - 10.4308/hjb.14.4.145
Subject(s) - biology , bacteria , botany , 16s ribosomal rna , plantlet , callus , intergenic region , explant culture , microbiology and biotechnology , genetics , gene , genome , in vitro
Endophytic bacteria live in plant host tissues without causing any symptoms. The aim of this study was to examine the indigenous endophytic bacteria on sugarcane plantlets produced from the young leaf cells by using tissue culture techniques. To detect the existence of endophytic bacteria in the plantlet tissue, a series of molecular method based on PCR were applied by using ribosomal intergenic spacer (RIS) primer followed by 16S rDNA partial sequence and single strand conformation polymorphism (SCCP). The results showed that the molecular method could detect the existence of bacteria in the tissues. Using the same methods, the bacteria were also found in other developmental stages of sugarcane (explants, differentiated tissues and callus)