Open AccessAnalytical Quality by Design Driven Development and Validation of UV-Visible Spectrophotometric Method for Quantification of Xanthohumol in Bulk and Solid Lipid Nanoparticles
Author(s) -
Harish Vancha,
Devesh Tewari,
Rajesh Kumar,
Pilli Govindaiah,
Sharfuddin Mohd,
Sachin Kumar Singh,
Monica Gulati
Publication year - 2023
Publication title -
turkish journal of pharmaceutical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.241
H-Index - 14
eISSN - 2148-6247
pISSN - 1304-530X
DOI - 10.4274/tjps.galenos.2022.05335
Subject(s) - detection limit , repeatability , solid lipid nanoparticle , chromatography , critical quality attributes , relative standard deviation , central composite design , box–behnken design , quality by design , materials science , chemistry , response surface methodology , nanoparticle , particle size , nanotechnology
Xanthohumol (XH) is a prenylated chalcone available naturally and has diverse pharmacological activities. It has some limitations in the physiological environment such as biotransformation and less gastrointestinal tract absorption. To overcome the limitations, we prepared nanoformulations [solid lipid nanoparticles (SLNs)] of XH. Therefore, an analytical method is required for the estimation of XH in the bulk nanoformulations, so we developed and validated a quality by design (QbD)-based ultraviolet (UV)-spectrophotometric method as per the International Conference of Harmonization (ICH) Q2 (R1) guidelines.