Open AccessA Novel Genotyping Method for Detection of the Muscarinic Receptor M1 Gene rs2067477 Polymorphism and Its Genotype/Allele Frequencies in a Turkish Population
Author(s) -
F. Özdemir,
Yağmur Kır,
Kenan Can Tok,
Bora Başkak,
Sinan Süzen
Publication year - 2020
Publication title -
turkish journal of pharmaceutical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.241
H-Index - 14
eISSN - 2148-6247
pISSN - 1304-530X
DOI - 10.4274/tjps.galenos.2019.46793
Subject(s) - genotyping , genotype , turkish population , biology , restriction fragment length polymorphism , allele , genetics , allele frequency , single nucleotide polymorphism , primer (cosmetics) , polymerase chain reaction , polymorphism (computer science) , microbiology and biotechnology , gene , chemistry , organic chemistry
Gene variation in the cholinergic muscarinic receptor 1 (CHRM1) has potential to become a candidate biomarker in the development of several disorders as well as drug response. In this study, a novel polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay was developed to determine the C to A single nucleotide polymorphism at position 267 in the CHRM1 gene.