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Cytotaxonomy of Trypanosoma cruzi (Chagas, 1909): Differentiation of T. cruzi I (TcI) and T. cruzi II (TcII) Genotypes Using Cytogenetic Markers
Author(s) -
Ana Beatriz Bortolozo de Oliveira,
A. R. Ribeiro,
Fernanda Fernandez Madeira,
Natália Regina Cesaretto,
João Aristeu da Rosa,
Maria Tercília Vilela de AzeredoOliveira,
Kaio Cesar Chaboli Alevi
Publication year - 2019
Publication title -
american journal of tropical medicine and hygiene
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.015
H-Index - 151
eISSN - 1476-1645
pISSN - 0002-9637
DOI - 10.4269/ajtmh.18-0650
Subject(s) - trypanosoma cruzi , chagas disease , biology , genotype , orcein , genetics , virology , gene , staining , parasite hosting , world wide web , computer science
Chagas disease is a public health problem caused by the Trypanosoma cruzi , and the T. cruzi I (TcI) and T. cruzi II (TcII) groups are considered important genotypes from the clinical point of view. Currently, the groups need to be molecularly analyzed for their identification; thus, we cytogenetically analyzed these groups with the objective of developing more accessible techniques for the characterization of these parasites. TcI and TcII groups were differentiated by nucleus characterization with lacto-acetic orcein (TcI-nucleus with positive heteropycnosis and TcII-nucleus with negative heteropycnosis), emphasizing the importance of the application of this technique for epidemiological and clinical studies of Chagas disease.

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