Open AccessPluripotent stem cell-derived neural stem cells: From basic research to applications
Author(s) -
Masahiro Otsu,
Takashi Nakayama,
Nozomu Inoue
Publication year - 2014
Publication title -
world journal of stem cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.907
H-Index - 18
ISSN - 1948-0210
DOI - 10.4252/wjsc.v6.i5.651
Subject(s) - induced pluripotent stem cell , reprogramming , embryonic stem cell , stem cell , neural stem cell , neurosphere , biology , regenerative medicine , adult stem cell , neuroscience , microbiology and biotechnology , cell , genetics , gene
Basic research on pluripotent stem cells is designed to enhance understanding of embryogenesis, whereas applied research is designed to develop novel therapies and prevent diseases. Attainment of these goals has been enhanced by the establishment of embryonic stem cell lines, the technological development of genomic reprogramming to generate induced-pluripotent stem cells, and improvements in vitro techniques to manipulate stem cells. This review summarizes the techniques required to generate neural cells from pluripotent stem cells. In particular, this review describes current research applications of a simple neural differentiation method, the neural stem sphere method, which we developed.