Open AccessDental stem cell-conditioned medium for tissue regeneration: Optimization of production and storage
Author(s) -
Batoul Chouaib,
Frédéric Cuisinier,
Pierre-Yves Collart-Dutilleul
Publication year - 2022
Publication title -
world journal of stem cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.907
H-Index - 18
ISSN - 1948-0210
DOI - 10.4252/wjsc.v14.i4.287
Subject(s) - mesenchymal stem cell , stem cell , dental pulp stem cells , paracrine signalling , tissue engineering , regeneration (biology) , regenerative medicine , microbiology and biotechnology , extracellular vesicle , in vivo , biomedical engineering , bioinformatics , chemistry , biology , medicine , biochemistry , microvesicles , microrna , receptor , gene
Mesenchymal stem cells (MSC) effects on tissue regeneration are mainly mediated by their secreted substances (secretome), inducing their paracrine activity. This Conditioned medium (CM), including soluble factors (proteins, nucleic acids, lipids) and extracellular vesicles is emerging as a potential alternative to cell therapy. However, the manufacturing of CM suffers from variable procedures and protocols leading to varying results between studies. Besides, there is no well-defined optimized procedure targeting specific applications in regenerative medicine.