Open AccessThree-dimensional cell culture systems as an in vitro platform for cancer and stem cell modeling
Author(s) -
Nipha Chaicharoenaudomrung,
Phongsakorn Kunhorm,
Parinya Noisa
Publication year - 2019
Publication title -
world journal of stem cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.907
H-Index - 18
ISSN - 1948-0210
DOI - 10.4252/wjsc.v11.i12.1065
Subject(s) - 3d cell culture , stem cell , cell culture , cell , cancer cell , cancer stem cell , microbiology and biotechnology , cancer , computational biology , biology , biochemistry , genetics
Three-dimensional (3D) culture systems are becoming increasingly popular due to their ability to mimic tissue-like structures more effectively than the monolayer cultures. In cancer and stem cell research, the natural cell characteristics and architectures are closely mimicked by the 3D cell models. Thus, the 3D cell cultures are promising and suitable systems for various proposes, ranging from disease modeling to drug target identification as well as potential therapeutic substances that may transform our lives. This review provides a comprehensive compendium of recent advancements in culturing cells, in particular cancer and stem cells, using 3D culture techniques. The major approaches highlighted here include cell spheroids, hydrogel embedding, bioreactors, scaffolds, and bioprinting. In addition, the progress of employing 3D cell culture systems as a platform for cancer and stem cell research was addressed, and the prominent studies of 3D cell culture systems were discussed.