Open AccessMendelian inheritance, genetic linkage, and genotypic disequilibrium for nine microsatellite loci in Cariniana estrellensis (Raddi) Kuntze (Lecythidaceae)
Author(s) -
Thaisa Y. K. Kubota,
A.M. Silva,
José Cambuim,
Anderson Antonio Correa da Silva,
Silvelise Pupin,
M.S. Silva,
Marcela Aparecida de Moraes,
M. L. T. Moraes,
Alexandre Magno Sebbenn
Publication year - 2017
Publication title -
genetics and molecular research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.356
H-Index - 48
ISSN - 1676-5680
DOI - 10.4238/gmr16029653
Subject(s) - biology , microsatellite , linkage disequilibrium , genetic diversity , mendelian inheritance , population , genetic structure , genetics , genetic variation , evolutionary biology , genotype , haplotype , allele , gene , demography , sociology
Cariniana estrellensis is one of the largest trees found in Brazilian tropical forests. The species is typical of advanced stages of succession, characteristic of climax forests, and essential in genetic conservation and environmental restoration plans. In this study, we assessed Mendelian inheritance, genetic linkage, and genotypic disequilibrium in nine microsatellite loci for a C. estrellensis population. We sampled and genotyped 285 adult trees and collected seeds from 20 trees in a fragmented forest landscape in Brazil. Based on maternal genotypes and their seeds, we found no deviation from the expected 1:1 Mendelian segregation and no genetic linkage between pairwise loci. However, for adults, genotypic disequilibrium was detected for four pairs of loci, suggesting that this result was not caused by genetic linkage. Based on these results, we analyzed microsatellite loci that are suitable for use in population genetic studies assessing genetic diversity, mating system, and gene flow in C. estrellensis populations.