Open AccessrbcS SRS4 promoter from Glycine max and its expression activity in transgenic tobacco
Author(s) -
Xiyan Cui,
Z Y Chen,
Lintao Wu,
X Q Liu,
Yan Dong,
F W Wang,
H Y Li
Publication year - 2015
Publication title -
genetics and molecular research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.356
H-Index - 48
ISSN - 1676-5680
DOI - 10.4238/2015.july.3.15
Subject(s) - nicotiana tabacum , transgene , transformation (genetics) , biology , genetically modified crops , gene , promoter , microbiology and biotechnology , glycine , agrobacterium , gene expression , regulatory sequence , beta glucuronidase , genetics , amino acid
The regulatory region of the ribulose-1,5-bisphosphate carboxylase small subunit gene SRS4 from soybean (Glycine max) was cloned using TAIL-PCR and general PCR, and named the rbcS promoter. The promoter was fused with the GUS gene and introduced into Nicotiana tabacum via Agrobacterium-mediated leaf disk transformation. In 4-week-old transgenic tobacco plants, the highest GUS expression levels were observed in the leaves, GUS activity was 7.13- and 7.40-fold higher in leaves than in stems and roots, respectively. Moreover, GUS activity was stimulated by light. In conclusion, spatial and light regulation of the soybean rbcS promoter was observed in N. tabacum, thus illustrating a leaf-specific and light-induced promoter.