Open AccessIsolation and characterization of polymorphic microsatellite markers in Bagarius yarrelli using RNA-Seq
Author(s) -
Min Du,
Liu Yh,
Niu Bz
Publication year - 2015
Publication title -
genetics and molecular research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.356
H-Index - 48
ISSN - 1676-5680
DOI - 10.4238/2015.december.8.21
Subject(s) - microsatellite , biology , locus (genetics) , genetics , catfish , population , allele , bonferroni correction , genetic structure , genetic marker , genetic variation , fish <actinopterygii> , gene , fishery , statistics , demography , mathematics , sociology
The yellow sisorid catfish (Bagarius yarrelli) is a vulnerable fish species. In this study, seven polymorphic microsatellite DNA markers for yellow sisorid catfish were described, using RNA-Seq methodology. In B. yarrelli (N = 44) from a Hekou wild population, allelic frequency, and observed and expected heterozygosities per locus varied from two to six, 0.0333 to 0.6793, and 0.0333 to 0.6004, respectively. One locus (Baya153) denoted notable separation from the Hardy-Weinberg equilibrium, after sequential Bonferroni correction (P < 0.05). The microsatellite markers described here will be useful for investigating population structure and genetic resource of B. yarrelli from different geographical locations.