Open AccessCloning and expression analysis of a stress-induced GmIMT1 gene in soybean (Glycine max)
Author(s) -
Wang Ht,
Na Guo,
Zhao Jm,
A Karthikeyan,
Dongfeng Xue,
Xue Cc,
Jian Xu,
Xu Zh,
Gai Jy,
Xiaobin Han
Publication year - 2014
Publication title -
genetics and molecular research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.356
H-Index - 48
ISSN - 1676-5680
DOI - 10.4238/2014.february.7.2
Subject(s) - abscisic acid , methyl jasmonate , biology , gene expression , gene , cloning (programming) , salicylic acid , transformation (genetics) , complementary dna , genetically modified crops , transgene , arabidopsis thaliana , botany , agrobacterium , microbiology and biotechnology , genetics , mutant , computer science , programming language
Here, we aimed to clone and identify the GmIMT1 gene related to the salt stress response in soybean. The full-length cDNA sequence of the GmIMT1 gene was amplified in soybean using degenerate primers of Mesembrythmum crystallium. To understand the stress response, the GmIMT1 gene was cloned and sequenced. Then, the expression vectors of the gene were constructed, and introduced into the model plant Arabidopsis thaliana through Agrobacterium mediated transformation, and the salt tolerance was analyzed in the transgenic plants. In addition, the expression patterns of GmIMT1 gene in soybean were analyzed. The expression was examined in different organs (roots, leaves, flower seeds, and stem) and under different stress conditions (drought, high salt, low temperature, salicylic acid, ethane, abscisic acid, and methyl jasmonate) by real-time fluorescent quantitative polymerase chain reaction analysis. The results showed that the root, leaves, and stems exhibited high level of GmIMT1 gene expression, whereas there was no expression in the seeds. In addition, the GmIMT1 gene expression was upregulated under all stress conditions. Overall, the results clearly indicate that GmIMT1 might be involved in multiple plant response pathways to the different environmental conditions. Furthermore transgenic plants exhibited higher salt-tolerance compared to wild type plants.