Open AccessEffect of CDH1 gene methylation on transforming growth factor (TGF-β)-induced epithelial-mesenchymal transition in alveolar epithelial cell line A549
Author(s) -
Y Y Liu,
Junyong Han,
Shaoze Lin,
Z Y Liu,
Wentan Jiang
Publication year - 2014
Publication title -
genetics and molecular research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.356
H-Index - 48
ISSN - 1676-5680
DOI - 10.4238/2014.february.13.13
Subject(s) - epithelial–mesenchymal transition , a549 cell , transforming growth factor , cdh1 , mesenchymal stem cell , cell culture , cell growth , cell , microbiology and biotechnology , cancer research , chemistry , biology , cadherin , transition (genetics) , biochemistry , gene , genetics
The aim of this study was to investigate the specific molecular mechanism of the transforming growth factor β (TGF-β)-induced epithelial-mesenchymal transition in a lung cancer cell line, and to provide new ideas for targeting therapy of lung cancer. A549 cells were treated with different concentrations of TGF-β and 5-aza-deoxycytidine (5-aza-dC). The morphological changes after the intervention were observed. The change in the expression of the epithelial marker E-cadherin (E-cad) was detected by Western blot. The proliferation of A549 cells was measured using the MTT assay. Cell movement and invasion capacity was evaluated with the cell scratch test and invasion test. TGF-β induced A549 cells to transform to a mesenchymal cell morphology and downregulated the expression of E-cad, and 5-aza-dC inhibited this phenomenon. Compared with the control group, the number of transmembrane cells was higher and cell migration was markedly increased in the experimental group with continued culture in the presence of 10 ng/mL TGF-β, showing significant differences (P < 0.05). CDH1 gene methylation is involved in TGF-β-induced epithelial-mesenchymal transition in the alveolar epithelial cell line A549.