Open AccessAgrobacterium tumefaciens-mediated genetic transformation of the Taxol-producing endophytic fungus Ozonium sp EFY21
Author(s) -
L Liu,
Yi Wei,
XuanWei Zhou,
Juan Lin,
Xiaofen Sun,
Kexuan Tang
Publication year - 2013
Publication title -
genetics and molecular research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.356
H-Index - 48
ISSN - 1676-5680
DOI - 10.4238/2013.august.12.7
Subject(s) - transformation (genetics) , agrobacterium tumefaciens , hygromycin b , biology , agrobacterium , fungus , expression vector , microbiology and biotechnology , botany , genetics , gene , recombinant dna
An efficient Agrobacterium tumefaciens-mediated genetic transformation method was successfully established for a newly isolated Taxol-producing fungus, Ozonium sp EFY21. A specific hygromycin B resistance expression vector, pCAMBIA1304'AN7-1, was constructed for fungal transformation. Key factors affecting transformation efficiency were thoroughly investigated and optimized. PCR amplification and Southern hybridization were used to verify the transformation events. This study should pave the way for future genetic modification studies of Ozonium sp EFY21.