Open AccessImproved Micropropagation in <i>Hybanthus enneaspermus</i> L. Muell
Author(s) -
Gandhi Premkumar,
N. Arumugam,
S. Muthuramkumar,
Govidaraju Varatharaju,
K Rajarathinam
Publication year - 2013
Publication title -
american journal of plant sciences
Language(s) - English
Resource type - Journals
eISSN - 2158-2750
pISSN - 2158-2742
DOI - 10.4236/ajps.2013.46144
Subject(s) - shoot , acclimatization , auxin , explant culture , micropropagation , botany , murashige and skoog medium , cytokinin , biology , chemistry , horticulture , in vitro , biochemistry , gene
Multiple shoots were induced on the cut ends of the leaf of Hybanthus enneaspermus on MS medium fortified with different concentration of cytokinins (KI and BAP) and in combination of KI and BAP. To enhance the multiple shoots induction, different types of auxins were used. IAA induced a maximum of 52.3 shoots per explants in combination with KI + BAP. The regenerated shoots show two different morphological leaves. Shoots are 100% rooted on half-strength MS medium supplemented with 2.46 μM·l-1 IBA. Rooted plantlets were successfully transferred to garden after acclimatization and they appeared morphologically normal.