Open AccessInterspecific Hybridization between <i>Arisaema sikokianum</i> and <i>A. serratum</i> (Araceae) Confirmed through Nuclear and Chloroplast DNA Comparisons
Author(s) -
Hiroshi Hayakawa,
Hidenori Hamachi,
Katsuhiko Matsuyama,
Yuko Muramatsu,
Yukio Minamiya,
Katsura Ito,
Jun Yokoyama,
Tatsuya Fukuda
Publication year - 2011
Publication title -
american journal of plant sciences
Language(s) - English
Resource type - Journals
eISSN - 2158-2750
pISSN - 2158-2742
DOI - 10.4236/ajps.2011.24061
Subject(s) - biology , chloroplast dna , gene , dna , nuclear dna , chloroplast , microbiology and biotechnology , genetics , mitochondrial dna
A morphologically intermediate plant between Arisaema sikokianum Franch. et Sav. and A. serratum (Thunb.) Schott has been newly found in Kochi Prefecture, Shikoku, Japan. The putative hybrid has the intermediate morphological characteristics of the parental species. Molecular analysis using PCR-RFLP of internal transcribed spacer (ITS) in nuclear DNA (nrDNA) indicates that the putative hybrid has a combined pattern of the two putative parent species. Moreover, the sequence result of chloroplast DNA (cpDNA) of the putative hybrid was identical to that of A. sikokianum. These results suggest that the putative hybrid is a hybrid between A. sikokianum and A. serratum and that it was formed by interactive gene exchanging via pollens from A. serratum to A. sikokianum. It is the first record of a hybrid between A. sikokianum and A. serratum