Open AccessHydralazine Hydrochloride: An Alternative Complexometric Reagent for Total Iron Spotometric Determination
Author(s) -
Arlan de Assis Gonsalves,
Cleônia Roberta Melo Araújo,
Cristiane Xavier Galhardo,
Marília Oliveira Fonseca Goulart,
Fabiane C. de Abreu
Publication year - 2011
Publication title -
american journal of analytical chemistry
Language(s) - English
Resource type - Journals
eISSN - 2156-8278
pISSN - 2156-8251
DOI - 10.4236/ajac.2011.27089
Subject(s) - chemistry , reagent , standard addition , calibration curve , ascorbic acid , detection limit , hydrochloride , chromatography , flow injection analysis , analytical chemistry (journal) , organic chemistry , food science
An alternative spectrophotometric method has been developed for total iron determination using flow injection analysis (FIA). The procedure is based on the coordination reaction between hydralazine and Fe2+ ions, which results in the formation of a purple complex monitored at 538 nm. For determination of total iron, Fe3+ ions were reduced using ascorbic acid. Under optimized conditions, a linear calibration graph (0.1 - 6.0 ?g?ml–1; n = 6) was obtained. The method allows LOD (3? of blank/slope = 0.06 ?g?ml–1) and LOQ (10? of blank/slope = 0.22 ?g?ml–1). The RSD ((s/ ) × 100) for a mixed standard containing 0.60 ?g?ml–1 Fe2+ and Fe3+ was 0.10% (n = 10). Recoveries of spiked samples were 94.3% - 106.0%. The analytical frequency was 60 h–1. The effect of possible interferences has been studied. The procedure was successfully applied for analysis of environmental samples. The real samples results were comparable with those obtained by the official method considering a paired t-test and 95% of confidence level