Open AccessLignocellulolytic activities of a novel strain of <i>Trichoderma harzianum</i>
Author(s) -
M. Rubeena,
Kannan Neethu,
Sreedharan Sajith,
Sasidharan Sreedevi,
Prakasan Priji,
Kizhakkepowathial Nair Unni,
M. K. Sarath Josh,
V. N. Jisha,
S. Pradeep,
Sailas Benjamin
Publication year - 2013
Publication title -
advances in bioscience and biotechnology
Language(s) - English
Resource type - Journals
eISSN - 2156-8502
pISSN - 2156-8456
DOI - 10.4236/abb.2013.42030
Subject(s) - cellulase , trichoderma harzianum , food science , chemistry , sodium nitrate , yeast extract , sucrose , lactose , carboxymethyl cellulose , fermentation , banana peel , cellulose , spore , botany , sodium , biochemistry , biology , organic chemistry , biological pest control
This study describes a novel dark-green spore producing strain of Trichoderma harzianum exhibiting higher activities of cellulase, hemicellulase and ligninase on specific plate assays. To assess the cellulase production in detail, basal salt medium (BSM) was supplemented with synthetic [carboxymethyl cellulose (CMC), glucose, sucrose, dextrose, lactose or maltose] and natural (flours of banana, banana peel, jack seed, potato or tapioca) carbon as well as nitrogen (yeast extract, beef extract, peptone, NaNO3 or NH4NO3) sources. Temperature and pH optima were 28?C and 4, respectively for the growth of the fungus in CMC- BSM with 146 U/ml cellulase activity. Flours of potato and banana supported comparable yields of cellulase to that of CMC (147 U/ml and 168 U/ml, respectively), while sodium nitrate was the preferred nitrogen source (150 U/ml). The water soluble yellowish-green pigment (a probable siderophore) extracted from the spores showed an absorption maximum at 414 nm. To comprise, this fungus shows the complete lignocellulolytic potential which offers great industrial significance, especially for the ethanol production from the lignocellulosic waste coupled with the production of a new pigment.