Open AccessAn In-silico Approach for Identifying Phytochemical Inhibitors Against Nervous Necrosis Virus (NNV) in Asian Sea Bass by Targeting Capsid Protein
Author(s) -
Sk Injamamul Islam,
Moslema Jahan Mou,
Saloa Sanjida,
Sarower Mahfuj
Publication year - 2022
Publication title -
genetics of aquatic organisms
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.185
H-Index - 3
ISSN - 2459-1831
DOI - 10.4194/ga487
Subject(s) - capsid , in silico , biology , virtual screening , pubchem , allium sativum , docking (animal) , sea bass , virus , virology , microbiology and biotechnology , biochemistry , botany , fishery , drug discovery , veterinary medicine , medicine , gene , fish <actinopterygii>
Nervous necrosis virus (NNV) is a devastating infectious pathogen for fish species with 100% mortality. To date, no specific drugs or vaccines have been developed that can prevent infections in aquaculture caused by NNV. It has been found that the NNV utilizes capsid protein to enter into the host cell in Asian sea bass and cause disease. In this study, we evaluated the inhibitory potential of Allium sativum compounds that have been reported to show antiviral activity against various pathogens. The capsid protein was modeled and the binding affinity of all the compounds was calculated with the docking approach and top 2 (PubChem CID: 122130381 and CID 12303662) inhibitory compounds were selected for further ADMET properties and DFT analysis. Both the geometry optimization and redocking of the two inhibitory compounds (PubChem CID: 122130381 and CID 12303662) showed a strong binding affinity of -8.2 and -8.0 kcal/mol, respectively with the capsid protein. The molecular dynamic simulation approach further validated the capsid protein – CID: 122130381 and capsid protein- CID 12303662 complex stability. In conclusion, this study deduces that these Allium sativum phytochemicals might act as significant inhibitors of the NNV in sea bass, which can be further validated experimentally.