Open AccessIsolation and Expression Analysis of cAMP-Dependent Protein Kinase and Adenylyl Cyclase-Associated Protein 1-like cDNAs in the Giant Tiger Shrimp Penaeus monodon
Author(s) -
Sirawut Klinbunga,
Kanchana Sittikankaew,
Sirikan Prasertlux,
Sirithorn Janpoom,
Puttawan Rongmung,
Wanwipa Ittarat,
Phimsucha Boonphimpapha,
Bavornlak Khamnamtong
Publication year - 2022
Publication title -
genetics of aquatic organisms
Language(s) - English
Resource type - Journals
ISSN - 2459-1831
DOI - 10.4194/ga480
Subject(s) - penaeus monodon , biology , broodstock , adenylyl cyclase , vitellogenin , shrimp , andrology , protein kinase c , medicine , endocrinology , vitellogenesis , microbiology and biotechnology , gene , oocyte , kinase , biochemistry , fishery , embryo , stimulation , fish <actinopterygii> , aquaculture
Characterization of genes exhibiting differential expression profiles during ovarian development is important for understanding reproductive maturation of the giant tiger shrimp (Penaeus monodon). Here, the partial cDNAs of P. monodon cAMP-dependent protein kinase, catalytic subunit 1 (PmPkaC1) and adenylyl cyclase-associated protein 1-like (PmCap1-l) were studied. They were more preferentially expressed in ovaries than testes of cultured juveniles and wild broodstock. PmPkaC1 mRNA in ovaries of non-ablated broodstock was significantly increased during vitellogenesis (P 0.05). Nevertheless, the expression of PmCap1-l was increased in ovaries treated with 0.1 and 10 μg/ml 17-20β-DHP at 24 hours post treatment (hpt, P<0.05).