Open AccessPhylogenetic of Marble Goby (Oxyeleotris marmorata Blkr.) in South Sumatra Based on Cytochrome C Oxidase Subunit I (COI) Gene
Author(s) -
Mochamad Syaifudin,
Dade Jubaedah,
Ferdinand Hukama Taqwa,
Ria Octaviani
Publication year - 2021
Publication title -
genetics of aquatic organisms
Language(s) - English
Resource type - Journals
ISSN - 2459-1831
DOI - 10.4194/ga433
Subject(s) - phylogenetic tree , domestication , goby , dna barcoding , biology , mitochondrial dna , cytochrome b , polymerase chain reaction , genetic distance , zoology , gene , evolutionary biology , genetics , genetic variation , fish <actinopterygii> , fishery
Marble goby (Oxyeleotris marmorata) in Indonesia, spread across Sumatra, Kalimantan and Papua. The purpose of this study was to utilize a sequence of mitochondrial DNA Cytochrome Oxidase Subunit I gene, to analyze the phylogenetic tree and genetic distance between cultured and captured populations. This research was conducted on March-August 2020. The methods used in barcoding species were DNA isolation, amplification using PCR (Polymerase Chain Reaction) and sequencing of the COI mtDNA gene. The domesticated samples (n=3) were collected from Gandus Fish Seed Center (GFSC), while the wild samples (n=3) were captured from the Musi River in Beruge Village, Babat Toman District, both in Musi Banyuasin Regency. The sequenced COI mtDNA gene fragments were obtained from the PCR method. Three samples performed good PCR results, while the other three didn’t amplify properly. After the editing process, the COI gene sequencing produced a nucleotide length of 610 bp. Based on the BLAST (Basic Local Alignment Search Tool), domesticated marble goby (OMD2, OMD3) was in the same cluster with marble goby from the Musi River (OMS3). The genetic distance indicated that two specimens of domesticated marble goby were 100% identical, while the wild (OMS3) indicated a genetic distance of 0.0066 to domesticated species.