Open AccessTh2 biased upper airway inflammation is associated with an impaired response to viral infection with Herpes simplex virus 1
Author(s) -
Feng Li,
Xiang Dong Wang,
Hans Nauwynck,
Gabriële Holtappels,
Luo Zhang,
Sebastian L. Johnston,
N. Papadopoulos,
Claus Bachert,
Nan Zhang
Publication year - 2016
Publication title -
rhinology (amsterdam. online)/rhinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.275
H-Index - 57
eISSN - 1996-8604
pISSN - 0300-0729
DOI - 10.4193/rhino15.213
Subject(s) - medicine , herpes simplex virus , immunology , tumor necrosis factor alpha , virus , inflammation , interferon , interleukin , cytokine , ex vivo , mucous membrane of nose , proinflammatory cytokine , in vivo , virology , biology , microbiology and biotechnology
Background: We aimed to elucidate possible differences in antiviral defense in chronic rhinosinusitis with nasal polyps (CRSwNP) mucosal tissue compared to healthy mucosal tissue (HMT) upon herpes simplex virus 1 (HSV1) exposure. Methodology: HMT and CRSwNP samples were infected with HSV1. We visualized the virus location by immunofluorescence and monitored invasion by a score. The mediators Interferon (IFN)-α, IFN-β, IFN-λ, IFN-γ, Interleukin (IL)-6, IL-1β, Tumor necrosis factor (TNF)-α, IL-17, IL-5, IL-10 were measured in culture supernatants at baseline and at 24 h, 48 h and 72 h after virus incubation. Results: CRSwNP mucosal tissue showed a significant deficit in IFN-γ and IL-17 release within 24 to 72 hours after infection in comparison to HMT, at the same time releasing significantly more pro-inflammatory cytokines including IL-1β and TNF-α. These findings were associated with significantly higher viral invasion scores at 48 and 72 h in CRSwNP mucosa compared to those for the HMT. Conclusions: We demonstrate for the first time in a human ex-vivo mucosal model that the inadequate response of CRSwNP may be associated with a deeper intrusion of viruses into the mucosal tissue, and may contribute to more and longer symptoms upon acute infection, but also to the persistence of inflammation in CRSwNP tissue.