Open AccessFipronil upregulates inflammatory cytokines and MUC5AC expression in human nasal epithelial cells
Author(s) -
So Young Kwak,
YouYoun Choi,
H.G. Na,
Chang Hoon Bae,
Suk-Jong Song,
Y.-D. Kim
Publication year - 2019
Publication title -
rhinology (amsterdam. online)/rhinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.275
H-Index - 57
eISSN - 1996-8604
pISSN - 0300-0729
DOI - 10.4193/rhin19.172
Subject(s) - fipronil , mapk/erk pathway , p38 mitogen activated protein kinases , cytokine , medicine , proinflammatory cytokine , signal transduction , kinase , inflammation , mucin , immunology , microbiology and biotechnology , biology , pathology , agronomy , pesticide
Airway inflammation and excessive mucin production are pathophysiological characteristics of airway diseases. Fipronil, a pesticide, is being extensively used in agriculture and veterinary medicine worldwide. However, this compound impairs immune function in non-target organisms. The present study aimed to evaluate the effect of fipronil on pro-inflammatory cytokine and mucus production and signalling pathways in human primary nasal METHODOLOGY: The effect of fipronil on pro-inflammatory cytokine and MUC5AC expression and the signalling pathway of fipronil were investigated using real-time PCR, enzyme immunoassays, immunofluorescence, and immunoblot analysis with specific inhibitors and small interfering RNA.