Open AccessImpacts of Interleukin-17 Neutralization on the Inflammatory Response in a Healing Ligament
Author(s) -
Anna E. B. Clements,
Connie S. Chamberlain,
Ellen M. Leiferman,
William L. Murphy,
Ray Vanderby
Publication year - 2017
Publication title -
journal of cytokine biology
Language(s) - English
Resource type - Journals
ISSN - 2576-3881
DOI - 10.4172/2576-3881.1000113
Subject(s) - neutralization , interleukin 1β , interleukin , inflammatory response , ligament , inflammation , immunology , medicine , anatomy , cytokine , antibody
In this study, we sought to improve ligament healing by modulating the inflammatory response after acute injury through the neutralization of Interleukin-17 (IL-17), which we hypothesized would decrease inflammatory cell infiltration and cytokine production. Administration of an Interleukin-17 neutralizing antibody (IL-17 NA) immediately following a rat medial collateral ligament (MCL) transection resulted in alterations in inflammatory cell populations and cytokine expression within the healing ligament, but did not reduce inflammation. Specifically, treatment resulted in a decrease in M2 (anti-inflammatory) macrophages, an increase in T cells, and an increase in the levels of IL-2, IL-6, and IL-12 in the MCL 7 days post injury. IL-17NA treatment, and subsequent immunomodulation, did not result in improved ligament healing, as measured by collagen composition and wound size.