Open AccessTranscriptional and Functional Plasticity Induced by Chronic Insulin Exposure in a Mast Cell-Like Basophilic Leukemia Cell Model
Author(s) -
Chad Jansen,
Mark Speck,
William E. Greineisen,
Kristina Maaetoft-Udsen,
Edward A. Cordasco,
Lori M. N. Shimoda,
Alexander J. Stokes,
Helen Turner
Publication year - 2017
Publication title -
journal of immunobiology
Language(s) - English
Resource type - Journals
ISSN - 2476-1966
DOI - 10.4172/2476-1966.1000135
Subject(s) - mast cell , biology , granule (geology) , downregulation and upregulation , microbiology and biotechnology , secretory vesicle , endocrinology , secretion , immunology , biochemistry , exocytosis , gene , paleontology
Secretory granules (SG) and lipid bodies (LB) are the primary organelles that mediate functional responses in mast cells. SG contains histamine and matrix-active proteases, while LB are reservoirs of arachidonic acid and its metabolites, precursors for rapid synthesis of eicosanoids such as LTC 4 . Both of these compartments can be dynamically or ontologically regulated, with metabolic and immunological stimuli altering lipid body content and granule numbers responding to contextual signals from tissue. We previously described that chronic in vitro or in vivo hyperinsulinemia expands the LB compartment with a concomitant loss of SG capacity, suggesting that this ratio is dynamically regulated. The objective of the current study is to determine if chronic insulin exposure initiates a transcriptional program that biases model mast cells towards a lipogenic state with accompanying loss of secretory granule biogenesis.