Open AccessModular Ultrasonic Lysis System for Rapid Nucleic Acid Extraction and Sample Transfer of Bacillus Spores
Author(s) -
Matthias Geißler
Publication year - 2012
Publication title -
journal of bioterrorism and biodefense
Language(s) - English
Resource type - Journals
ISSN - 2157-2526
DOI - 10.4172/2157-2526.1000119
Subject(s) - bacillus anthracis , nucleic acid , lysis , dna extraction , spore , dna , chromatography , modular design , microbiology and biotechnology , biology , chemistry , computer science , bacteria , biochemistry , polymerase chain reaction , genetics , gene , operating system
This paper describes the design, functioning and use of an ultrasonic modular system intended for rapid extraction and fragmentation of DNA from microbial organisms following sample collection in the field. PCR assessment of the DNA extracts revealed that the system can disrupt Bacillus atrophaeus spores, a simulant for Bacillus anthracis, in less than 1 min, providing a DNA yield equivalent to that of a commercial nucleic acid extraction method. Simulation of the transfer from a contaminated to a secure area confirmed that the sample remained confined within the module while the exterior surface can be decontaminated through immersion in a disinfectant solution.Peer reviewed: YesNRC publication: Ye