Open AccessSpADS and SNAP-NAPPA Microarrays towards Biomarkers Identification in Humans: Background Subtraction in Mass Spectrometry with E.coli Cell Free Expression System
Author(s) -
Claudio Nicolini,
Rosanna Spera,
Eugenia Pechkova
Publication year - 2015
Publication title -
journal of molecular biomarkers and diagnosis
Language(s) - English
Resource type - Journals
ISSN - 2155-9929
DOI - 10.4172/2155-9929.1000214
Subject(s) - identification (biology) , dna microarray , computational biology , mass spectrometry , cell , microarray , biology , chemistry , chromatography , gene expression , genetics , gene , botany
We present a useful approach towards for biomarkers identification in an innovative self-assembling protein microarray based on “Nucleic Acid Programmable Protein Array” (NAPPA) and SNAP tag coupled to E.coli cell free expression system. This approach prove capable to resolve the “background” problem associated to the above label free detection system for the identification of proteins and of protein-protein interaction in humans that could become used in clinical practice