Open AccessSynthesizing a Cellulase like Chimeric Protein by Recombinant Molecular Biology Techniques
Author(s) -
Hirendra Nath Banerjee,
Christopher Krauss,
Valerie Smith,
Kelly Mahaffey,
Ava Boston
Publication year - 2016
Publication title -
journal of bioprocessing and biotechniques
Language(s) - English
Resource type - Journals
ISSN - 2155-9821
DOI - 10.4172/2155-9821.1000285
Subject(s) - cellulase , cellulosic ethanol , cellulose , recombinant dna , biofuel , microbiology and biotechnology , molecular cloning , chemistry , bioreactor , rendering (computer graphics) , cloning (programming) , biology , biochemistry , gene , botany , gene expression , computer science , computer graphics (images) , programming language
In order to meet the Renewable Fuels Standard demands for 30 billion gallons of biofuels by the end of 2020, new technologies for generation of cellulosic ethanol must be exploited. Breaking down cellulose by cellulase enzyme is very important for this purpose but this is not thermostable and degrades at higher temperatures in bioreactors. Towards creation of a more ecologically friendly method of rendering bioethanol from cellulosic waste, we attempted to produce recombinant higher temperature resistant cellulases for use in bioreactors. The project involved molecular cloning of genes for cellulose-degrading enzymes based on bacterial source, expressing the recombinant proteins in E. coli and optimizing enzymatic activity. We were able to generate in vitro bacterial expression systems to produce recombinant His-tag purified protein which showed cellulase like activity.