Open AccessCross-Platform Urine Metabolomics of Experimental Hyperglycemia in Type 2 Diabetes
Author(s) -
Liesbet Temmerman
Publication year - 2013
Publication title -
journal of diabetes and metabolism
Language(s) - English
Resource type - Journals
ISSN - 2155-6156
DOI - 10.4172/2155-6156.s6-002
Subject(s) - medicine , diabetes mellitus , urine , metabolomics , type 2 diabetes , omics , glycosuria , bioinformatics , endocrinology , biology
Hyperglycemia causes diabetic nephropathy, a condition for which there are no specific diagnostic markers thatpredict progression to renal failure. Here we describe a multiplatform metabolomic analysis of urine from individualswith type 2 diabetes, collected before and immediately following experimental hyperglycemia. We used targetednuclear magnetic resonance spectroscopy (NMR), liquid chromatography - mass spectrometry (LC-MS) and gaschromatography - MS (GC-MS) to identify markers of hyperglycemia. Following optimization of data normalisation andstatistical analysis, we identified a reproducible NMR and LC-MS based urine signature of hyperglycemia. Significantincreases of alanine, alloisoleucine, isoleucine, leucine, N-isovaleroylglycine, valine, choline, lactate and taurine anddecreases of arginine, gamma-aminobutyric acid, hippurate, suberate and N-acetylglutamate were observed. GC-MSanalysis identified a number of metabolites differentially present in post-glucose versus baseline urine, but these could not be identified using current metabolite libraries. This analysis is an important first step towards identifying biomarkers of early-stage diabetic nephropathy