Open AccessA High Sensitive Nested PCR for Toxoplasma gondii Detection in Animal and Food Samples
Author(s) -
María Vitale
Publication year - 2013
Publication title -
journal of microbial and biochemical technology
Language(s) - English
Resource type - Journals
ISSN - 1948-5948
DOI - 10.4172/1948-5948.1000097
Subject(s) - toxoplasma gondii , nested polymerase chain reaction , biology , virology , veterinary medicine , polymerase chain reaction , genetics , gene , medicine , antibody
Toxoplasma gondii is a major food and waterborne transmitted parasite world-wide. The tissues and meat\udsamples of many warm blooded animals can contain tissues cysts from chronic toxoplasmosis. Water and vegetable\udcan be contaminated by the parasitic oocysts shed through the feces of infected cats, representing the definitive\udhost of the parasite.\udA sensitive PCR for Toxoplasma gondii detection is described. The first step amplified the region between the\ud28S and 18S rDNA in the closely related T. gondii and Neospora caninum; RFLP analysis distinguished the DNA\udfrom the two morphologically identical parasites. Although N. caninum is not involved in human transmission, so far,\udit is important for animal health since is a major responsible for abortion in cattle.\udThe nested PCR was used in a dilution assay in pork sausage samples spiked with T. gondii parasitic DNA. The\udanalysis showed that up to 200fg equivalent to two single parasites only, could be detected. Similar detection limit\udfor T. gondii can be obtained with real-time PCRs, but real time methods need special consumables and expensive\udequipment