Open AccessL(+) Lactic Acid Production by New Lactobacillus Rhamnosus B 103
Author(s) -
Cristian Jacques Bolner de Lima,
Luciana Fontes Coelho,
Gervásio Paulo da Silva,
Georgina L. M. Alvarez,
Jonas Contiero
Publication year - 2010
Publication title -
journal of microbial and biochemical technology
Language(s) - English
Resource type - Journals
ISSN - 1948-5948
DOI - 10.4172/1948-5948.1000025
Subject(s) - lactobacillus rhamnosus , lactic acid , production (economics) , lactobacillus , microbiology and biotechnology , biology , food science , bacteria , fermentation , genetics , economics , macroeconomics
L(+) Lactic acid fermentation was studied by Lactobacillus rhamnosus sp. under the effects of pH control and a lowcost nutritional medium (sugarcane juice and corn steep liquor-CSL). Central composite design (CCD) was employed to determine maximum lactic acid production at optimum values for process variables and a satisfactory fit model was realized. Statistical analysis of the results showed that the linear and quadratic terms of two variables (sugarcane juice and pH) had significant effects. The interactions between the three variables were found to contribute to the response at a significant level. A second-order polynomial regression model estimated that the maximum lactic acid production of 86.36 g/L was obtained when the optimum values of sucrose, CSL and pH were 112.65 g/L, 29.88 g/L and 6.2, respectively. Verification of the optimization showed that L(+) lactic acid production was of 85.06 g/L. Under these conditions, Y P/S and Q P values of 0.85 g/g and 1.77 g/Lh, respectively, were obtained after 48 h fermentation, with a maximal productivity of 2.2 g/L h at 30 h of process. © 2010 de Lima CJB, et al