Open AccessExpression in Escherichia coli and Purification of Folded rDer p 20, the Arginine Kinase From Dermatophagoides pteronyssinus: A Possible Biomarker for Allergic Asthma
Author(s) -
Eszter Sarzsinszky,
Christian Lupinek,
Susanne Vrtala,
HueyJy Huang,
Gerhard Hofer,
Walter Keller,
Kuan Wei Chen,
Carmen Panaitescu,
Yvonne Resch,
Petra Zieglmayer,
René Zieglmayer,
Patrick Lemell,
Friedrich Horak,
Michael Duchêne,
Rudolf Valenta
Publication year - 2021
Publication title -
allergy, asthma and immunology research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.356
H-Index - 45
eISSN - 2092-7363
pISSN - 2092-7355
DOI - 10.4168/aair.2021.13.1.154
Subject(s) - arginine kinase , escherichia coli , biomarker , immunology , asthma , arginine , microbiology and biotechnology , allergic asthma , arginase , kinase , pyroglyphidae , allergy , chemistry , biology , biochemistry , allergen , gene , amino acid , house dust mite
Arginine kinase (AK) was first identified as an allergen in the Indian-meal moth and subsequently shown to occur as allergen in various invertebrates and shellfish. The cDNA coding for AK from the house dust mite (HDM) species Dermatophagoides pteronyssinus , Der p 20, has been isolated, but no recombinant Der p 20 (rDer p 20) allergen has been produced and characterized so far. We report the expression of Der p 20 as recombinant protein in Escherichia coli . rDer p 20 was purified and shown to be a monomeric, folded protein by size exclusion chromatography and circular dichroism spectroscopy, respectively. Using AK-specific antibodies, Der p 20 was found to occur mainly in HDM bodies, but not in fecal particles. Thirty percent of clinically well-characterized HDM allergic patients (n = 98) whose immunoglobulin E (IgE) reactivity profiles had been determined with an extensive panel of purified HDM allergens (Der f 1, 2; Der p 1, 2, 4, 5, 7, 10, 11, 14, 15, 18, 21, 23 and 37) showed IgE reactivity to Der p 20. IgE reactivity to Der p 20 was more frequently associated with lung symptoms. AKs were detected in several invertebrates with specific antibodies and Der p 20 showed IgE cross-reactivity with AK from shrimp ( Litopenaeus vannamei ). Thus, Der p 20 is a cross-reactive HDM allergen and may serve as a diagnostic marker for HDM-induced lung symptoms such as asthma.