Open AccessQuantitative bioanalysis of strontium in human serum by inductively coupled plasma-mass spectrometry
Author(s) -
Srikanth Somarouthu,
Jayoung Ohh,
Jonathan Shaked,
Robert L. Cunico,
Gerald J. Yakatan,
Suzana Corritori,
Joseph A. Tami,
Erik D. Foehr
Publication year - 2015
Publication title -
future science oa
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.825
H-Index - 23
ISSN - 2056-5623
DOI - 10.4155/fso.15.76
Subject(s) - bioanalysis , inductively coupled plasma mass spectrometry , mass spectrometry , chemistry , chromatography , strontium , organic chemistry
Aim: A bioanalytical method using inductively-coupled plasma-mass spectrometry to measure endogenous levels of strontium in human serum was developed and validated. Results & methodology: This article details the experimental procedures used for the method development and validation thus demonstrating the application of the inductively-coupled plasma-mass spectrometry method for quantification of strontium in human serum samples. The assay was validated for specificity, linearity, accuracy, precision, recovery and stability. Significant endogenous levels of strontium are present in human serum samples ranging from 19 to 96 ng/ml with a mean of 34.6 ± 15.2 ng/ml (SD). Discussion & conclusion: Calibration procedures and sample pretreatment were simplified for high throughput analysis. The validation demonstrates that the method was sensitive, selective for quantification of strontium ( 88 Sr) and is suitable for routine clinical testing of strontium in human serum samples.