Open AccessSphingolipid biosynthetic pathway is crucial for growth, biofilm formation and membrane integrity of Scedosporium boydii
Author(s) -
Rodrigo Rollin-Pinheiro,
Victor Pereira Rochetti,
Mariana Ingrid Dutra da Silva Xisto,
Livia Cristina Liporagi-Lopes,
Beatriz Cabral Bastos,
Antonella Rella,
Ashutosh Singh,
Sônia Rozental,
Maurizio Del Poeta,
Eliana BarretoBergter
Publication year - 2019
Publication title -
future medicinal chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.708
H-Index - 69
eISSN - 1756-8927
pISSN - 1756-8919
DOI - 10.4155/fmc-2019-0186
Subject(s) - sphingolipid , biology , antifungal drug , microbiology and biotechnology , virulence , antifungal , biofilm , biochemistry , bacteria , gene , genetics
Aim: Glycosphingolipids are conserved lipids displaying a variety of functions in fungal cells, such as determination of cell polarity and virulence. They have been considered as potent targets for new antifungal drugs. The present work aimed to test two inhibitors, myriocin and DL-threo-1-Phenyl-2-palmitoylamino-3-morpholino-1-propanol, in Scedosporium boydii , a pathogenic fungus which causes a wide range of disease. Materials & methods: Mass spectrometry, microscopy and cell biology approaches showed that treatment with both inhibitors led to defects in fungal growth and membrane integrity, and caused an increased susceptibility to the current antifungal agents. Conclusion: These data demonstrate the antifungal potential of drugs inhibiting sphingolipid biosynthesis, as well as the usefulness of sphingolipids as promising targets for the development of new therapeutic options.